|
KMID : 0606920120200030352
|
|
Biomolecules & Therapeutics
2012 Volume.20 No. 3 p.352 ~ p.356
|
|
|
Controversial Effect of Ethanol Irrespective of Kinases Inhibition on the Agonist-Dependant Vasoconstriction
|
|
Je Hyun-Dong
Kim Hyeong-Dong
Park June-Hong
|
|
|
Abstract
|
|
|
|
The present study was undertaken to determine whether ethanol influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Ethanol significantly inhibited thromboxane A2 mimetic-induced contraction with intact endothelial function, but there was no relaxation on thromboxane A2 mimetic U-46619-induced contraction irrespective of endothelium suggesting that the pathway such as Rho-kinase activation, Ca2+ entry or thin filament regulation was not affected. In addition, ethanol didn’t decrease thromboxane A2 mimetic-induced increase of phospho-myosin phosphatase targeting subunit protein 1 (pMYPT1) or pERK1/2. Interestingly, ethanol didn’t inhibit significantly phorbol ester-induced contraction in denuded muscles suggesting that thin filament regulation is less important on the ethanol-induced regulation in the muscle than endothelial NO synthesis. In conclusion, this study provides the evidence and possible related mechanism concerning the effect of ethanol on the agonist-dependent contraction in rat aortic rings with regard to endothelial function.
|
|
|
KEYWORD
|
|
|
Ethanol, Phorbol ester, Rho-kinase, Thromboxane A2 mimetic, Vasodilation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|